Integration and interoperability between laboratory systems –or lack thereof—remains a challenge for those performing next-generation sequencing (NGS) or other genomics studies.[i] To address this challenge, we developed version 2.2 of the integration between BaseSpace Clarity LIMS and the NovaSeq 6000 instrument. This integration now supports the NovaSeq S1 flow cell.
The NovaSeq S1 flow cell delivers up to 0.5TB of output in two days and is ideally suited for high-intensity sequencing applications. Users can now sequence up to 8 human genomes or 80 exomes per run in approximately 24 hours.[ii] And now, users of both Basespace Clarity LIMS and NovaSeq 6000 instrument can access this out-of-the box integration to quickly get up and running with their system.
The NovaSeq 6000 version 2.0 Workflow in BaseSpace Clarity LIMS that supports the integration version 2.2.1
The integration helps users track samples throughout the workflow. Specifically, it:
- Supports S1, S2, and S4 flow cells per sample
- Supports different applications on the same flow cell
- Calculates samples and reagents volumes based on the flow cell type
- Creates an output file for use with liquid handling robots
- Validates every step in the workflow
The integration also tracks sequencing run information in BaseSpace Clarity LIMS to help with troubleshooting or trending:
- Run recipe files (JSON) are automatically generated to set up and initiate the run
- Sample sheets, which are compatible with BaseSpace Sequence Hub and bcl2fastq v 2.19, are automatically generated and placed directly on the NovaSeq 6000 instrument
- Sequencing run are tracked and run metrics are parsed per lane and per flow cell
If you have questions about this integration, please contact Technical Support.
For Research Use Only. Not for use in diagnostic procedures.
[i] Next-Generation Sequencing Informatics: Challenges and … http://www.bing.com/cr?IG=74008A18392242E59F11965A936C0331&CID=1B0873003B0C6EB91053783A3A0A6F0E&rd=1&h=qZ8eqx6ov_OxkAzDtTWfrbsSZM2WP_pCoQuO66f-AVI&v=1&r=http%3a%2f%2fwww.archivesofpathology.org%2fdoi%2f10.5858%2farpa.2015-0507-RA&p=DevEx,5067.1. Accessed November 14, 2017.
[ii] Illumina.com. (2017). Illumina Releases NovaSeq S4 Flow Cell and NovaSeq Xp Workflow. [online] Available at: https://www.illumina.com/company/news-center/press-releases/2017/2308795.html [Accessed 16 Nov. 2017].