Velvet de novo Assembly FastQC
Both applications are currently available for all users and were built using the BaseSpace Native App Engine by our internal R&D groups. These two applications are also the first BaseSpace Labs Apps of many more to come, the concept behind BaseSpace Labs Apps is explained in more detail below.
BaseSpace Labs Apps are Illumina’s internally developed applications that extend the functionality within BaseSpace. Some BaseSpace Labs applications will be experimental or research focused, while others will be used as a step in a greater workflow. The Apps are reviewed regularly by our team and put through the same review process as third-party apps.
BaseSpace Labs Apps are developed using an accelerated development process in order to make them available to BaseSpace users faster than the BaseSpace Core Apps. It is important to note that, unlike BaseSpace Core Apps, BaseSpace Labs Apps are not officially supported by Illumina Customer Service. Support for BaseSpace Labs applications is provided at the developer’s discretion and the apps are provided as-is without any warranty of any kind.
The FastQC app can be used to provide a quality assessment of the sequence data generated using Illumina sequencers. FastQC for BaseSpace is based on the FastQC software developed by the Bioinformatics Group at the Babraham Institute. It provides a modular set of analyses which can be used quickly to assess if there are any problems with the sequencing data before doing any additional analysis.
The above figure shows an example output from the FastQC app depicting the quality score across all bases at a given position in the reads. For an example of additional output generated by FastQC, please view this FastQC demo project.
The Velvet de novo Assembly app is a de novo assembly pipeline for bacterial samples using the Velvet assembler. One of the key features of this app is that it has an adapter trimming protocol that has been optimized for the Nextera Mate-Pair library prep kit. An application note describing the de novo assembly of 9 different bacterial using the Velvet de novo Assembly app can be found here. In many cases, a single contig representing the entire bacterial genome can be assembled. The figure below is an example of the output generate by the Velvet de novo Assembly app.
Example output generated by the Velvet de novo Assembly can be found here. We hope you enjoy the FastQC and Velvet de novo Assembly apps. For any questions, feedback, or feature requests for these applications, please send an email to firstname.lastname@example.org and include the name of the application. Thank you!